Review



lncrna + mrna mouse gene expression microarray v1.0, 4x180k chip  (CapitalBio Corporation)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    CapitalBio Corporation lncrna + mrna mouse gene expression microarray v1.0, 4x180k chip
    Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between <t>microarray</t> data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐ p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2
    Lncrna + Mrna Mouse Gene Expression Microarray V1.0, 4x180k Chip, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+lncrna+mrna+v1%2E0+microarray/agilent+mouse+lncrna+chip/pmc06787463-34-1-13
    Average 90 stars, based on 1 article reviews
    lncrna + mrna mouse gene expression microarray v1.0, 4x180k chip - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout"

    Article Title: Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.14584

    Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐ p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2
    Figure Legend Snippet: Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐ p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2

    Techniques Used: Expressing, Infection, Selection, Plasmid Preparation, Negative Control, Western Blot, Comparison, Microarray, Transformation Assay

    Construction of KP cell lines with Kras gene disruption and identification of Kras related lncRNAs in KP cells. A, Messy peak figure reporting the results of DNA sequencing showed mutations induced by Cas9/gRNA in the Kras guide RNA. The stable Cas9 expression KP cells (KP‐Cas9) were developed by pCDH‐bla‐Cas9 lentivirus infection and selection with 10 μg/mL blasticidin. KP‐Cas9 cells were further infected by the Kras ‐targeting guide RNA expression lentivirus and selected by 4 μg/mL puromycin, lenti‐guide empty vector lentivirus as negative control. The DNA fragment containing the Kras guide RNA binding site was amplified by using PCR with the genomic DNA of guide RNA‐infected KP‐cas9 cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by Kras . Among the selected 210 lncRNAs, qPCR results determined 33 lncRNAs, whose expression was altered by the Kras deletion in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP‐Kras‐guide cells/ KP‐guide‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2
    Figure Legend Snippet: Construction of KP cell lines with Kras gene disruption and identification of Kras related lncRNAs in KP cells. A, Messy peak figure reporting the results of DNA sequencing showed mutations induced by Cas9/gRNA in the Kras guide RNA. The stable Cas9 expression KP cells (KP‐Cas9) were developed by pCDH‐bla‐Cas9 lentivirus infection and selection with 10 μg/mL blasticidin. KP‐Cas9 cells were further infected by the Kras ‐targeting guide RNA expression lentivirus and selected by 4 μg/mL puromycin, lenti‐guide empty vector lentivirus as negative control. The DNA fragment containing the Kras guide RNA binding site was amplified by using PCR with the genomic DNA of guide RNA‐infected KP‐cas9 cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by Kras . Among the selected 210 lncRNAs, qPCR results determined 33 lncRNAs, whose expression was altered by the Kras deletion in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP‐Kras‐guide cells/ KP‐guide‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2

    Techniques Used: Disruption, DNA Sequencing, Expressing, Infection, Selection, RNA Expression, Plasmid Preparation, Negative Control, RNA Binding Assay, Amplification, Comparison, Microarray, Transformation Assay

    Identification of hypoxia‐related lncRNAs in KP cells. A, KP cells were incubated under hypoxia with 1% O 2 and normoxia (20% O 2 ). Immunoblot (left) was used to detect HIF1α protein levels and was quantified by measuring the grey value of the bands. Data are shown as means ± SD. All P values are from two‐sided t tests. B, Comparison between microarray data and qPCR results of lncRNAs associated with hypoxia. The qPCR result validated the 13 out of 210 selected lnRNAs were regulated by hypoxia, whose expression exhibited same expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP cells cultured in hypoxia/KP cells cultured in normoxia in qPCR results) in expression across the three lung adenocarcinoma tissues and KP cells cultured in hypoxia. Mean ± SD are shown, n = 3 or 2
    Figure Legend Snippet: Identification of hypoxia‐related lncRNAs in KP cells. A, KP cells were incubated under hypoxia with 1% O 2 and normoxia (20% O 2 ). Immunoblot (left) was used to detect HIF1α protein levels and was quantified by measuring the grey value of the bands. Data are shown as means ± SD. All P values are from two‐sided t tests. B, Comparison between microarray data and qPCR results of lncRNAs associated with hypoxia. The qPCR result validated the 13 out of 210 selected lnRNAs were regulated by hypoxia, whose expression exhibited same expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP cells cultured in hypoxia/KP cells cultured in normoxia in qPCR results) in expression across the three lung adenocarcinoma tissues and KP cells cultured in hypoxia. Mean ± SD are shown, n = 3 or 2

    Techniques Used: Incubation, Western Blot, Comparison, Microarray, Expressing, Transformation Assay, Cell Culture

    Confirmation of the p53‐ , Kras‐ or hypoxia‐ associated lncRNAs. A, Overlapped differentially expressed lncRNAs. B, Among the total 52 lncRNAs identified to be associated with p53 , Kras or hypoxia, 19 lncRNAs, with expression change fold greater than 3.5 in the microarray results, were selected to further confirmed by using quantitative RT‐PCR in another group of mouse lung adenocarcinoma tissues (6 lung adenocarcinoma samples and 6 normal lung tissues). The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues). The validation results of the 19 lncRNAs indicated that the microarray data correlated well with the qPCR results. Mean ± SD are shown, n = 3 or 6. C, Long noncoding RNA (lncRNA)‐lncRNA co‐expression network (LLCN) related to the 19 selected lncRNAs was constructed. Among the 19 lncRNAs, 6 lncRNAs had no significant co‐expression with other lncRNAs. The circles represent nodes of differential expressed lncRNAs (DE lncRNAs). The nodes in green colour represent the 13 remained lncRNAs. The LLCN contained a total of 4086 lncRNAs with 19 595 edges between them
    Figure Legend Snippet: Confirmation of the p53‐ , Kras‐ or hypoxia‐ associated lncRNAs. A, Overlapped differentially expressed lncRNAs. B, Among the total 52 lncRNAs identified to be associated with p53 , Kras or hypoxia, 19 lncRNAs, with expression change fold greater than 3.5 in the microarray results, were selected to further confirmed by using quantitative RT‐PCR in another group of mouse lung adenocarcinoma tissues (6 lung adenocarcinoma samples and 6 normal lung tissues). The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues). The validation results of the 19 lncRNAs indicated that the microarray data correlated well with the qPCR results. Mean ± SD are shown, n = 3 or 6. C, Long noncoding RNA (lncRNA)‐lncRNA co‐expression network (LLCN) related to the 19 selected lncRNAs was constructed. Among the 19 lncRNAs, 6 lncRNAs had no significant co‐expression with other lncRNAs. The circles represent nodes of differential expressed lncRNAs (DE lncRNAs). The nodes in green colour represent the 13 remained lncRNAs. The LLCN contained a total of 4086 lncRNAs with 19 595 edges between them

    Techniques Used: Expressing, Microarray, Quantitative RT-PCR, Transformation Assay, Biomarker Discovery, Construct



    Similar Products

    lncrna + mrna mouse gene expression microarray v1.0, 4x180k chip  (CapitalBio Corporation)
    90
    CapitalBio Corporation lncrna + mrna mouse gene expression microarray v1.0, 4x180k chip
    Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between <t>microarray</t> data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐ p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2
    Lncrna + Mrna Mouse Gene Expression Microarray V1.0, 4x180k Chip, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+lncrna+mrna+v1%2E0+microarray/agilent+mouse+lncrna+chip/pmc06787463-34-1-13
    Average 90 stars, based on 1 article reviews
    lncrna + mrna mouse gene expression microarray v1.0, 4x180k chip - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    mouse lncrna+mrna v1.0 microarray  (CapitalBio Corporation)
    90
    CapitalBio Corporation mouse lncrna+mrna v1.0 microarray
    Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between <t>microarray</t> data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐ p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2
    Mouse Lncrna+Mrna V1.0 Microarray, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+lncrna+mrna+v1%2E0+microarray/agilent+mouse+lncrna+chip/pm29466788-79-11-10
    Average 90 stars, based on 1 article reviews
    mouse lncrna+mrna v1.0 microarray - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐ p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout

    doi: 10.1111/jcmm.14584

    Figure Lengend Snippet: Construction of P53 re‐expression KP cells and identification of lncRNAs regulated by P53. A, P53 was efficiently re‐expressed in the primary KP cells. The P53‐re‐expressed KP cells (KP‐P53) were developed by PCDH‐Flag‐P53 lentiviral infection and puromycin selection （4 μg/mL）, using PCDH‐Flag empty vector lentivirus as negative control. Western blot analysis was used to analyse the expression of P53 in lysates from KP cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by P53. Among the selected 210 lncRNAs, qPCR results determined 11 lncRNAs, whose expression was altered by the re‐expression of P53 in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data or KP‐ p53 cells/KP‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2

    Article Snippet: For microarray analysis, lncRNA + mRNA mouse Gene Expression Microarray v1.0, 4x180K chip (CapitalBio Corp), was employed and conducted by CapitalBio Corp according to the manufacturer's protocols.

    Techniques: Expressing, Infection, Selection, Plasmid Preparation, Negative Control, Western Blot, Comparison, Microarray, Transformation Assay

    Construction of KP cell lines with Kras gene disruption and identification of Kras related lncRNAs in KP cells. A, Messy peak figure reporting the results of DNA sequencing showed mutations induced by Cas9/gRNA in the Kras guide RNA. The stable Cas9 expression KP cells (KP‐Cas9) were developed by pCDH‐bla‐Cas9 lentivirus infection and selection with 10 μg/mL blasticidin. KP‐Cas9 cells were further infected by the Kras ‐targeting guide RNA expression lentivirus and selected by 4 μg/mL puromycin, lenti‐guide empty vector lentivirus as negative control. The DNA fragment containing the Kras guide RNA binding site was amplified by using PCR with the genomic DNA of guide RNA‐infected KP‐cas9 cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by Kras . Among the selected 210 lncRNAs, qPCR results determined 33 lncRNAs, whose expression was altered by the Kras deletion in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP‐Kras‐guide cells/ KP‐guide‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout

    doi: 10.1111/jcmm.14584

    Figure Lengend Snippet: Construction of KP cell lines with Kras gene disruption and identification of Kras related lncRNAs in KP cells. A, Messy peak figure reporting the results of DNA sequencing showed mutations induced by Cas9/gRNA in the Kras guide RNA. The stable Cas9 expression KP cells (KP‐Cas9) were developed by pCDH‐bla‐Cas9 lentivirus infection and selection with 10 μg/mL blasticidin. KP‐Cas9 cells were further infected by the Kras ‐targeting guide RNA expression lentivirus and selected by 4 μg/mL puromycin, lenti‐guide empty vector lentivirus as negative control. The DNA fragment containing the Kras guide RNA binding site was amplified by using PCR with the genomic DNA of guide RNA‐infected KP‐cas9 cells. B, Comparison between microarray data and qPCR results of lncRNAs regulated by Kras . Among the selected 210 lncRNAs, qPCR results determined 33 lncRNAs, whose expression was altered by the Kras deletion in KP cells and exhibited opposite expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP‐Kras‐guide cells/ KP‐guide‐NC cells in qPCR results). Mean ± SD are shown, n = 3 or 2

    Article Snippet: For microarray analysis, lncRNA + mRNA mouse Gene Expression Microarray v1.0, 4x180K chip (CapitalBio Corp), was employed and conducted by CapitalBio Corp according to the manufacturer's protocols.

    Techniques: Disruption, DNA Sequencing, Expressing, Infection, Selection, RNA Expression, Plasmid Preparation, Negative Control, RNA Binding Assay, Amplification, Comparison, Microarray, Transformation Assay

    Identification of hypoxia‐related lncRNAs in KP cells. A, KP cells were incubated under hypoxia with 1% O 2 and normoxia (20% O 2 ). Immunoblot (left) was used to detect HIF1α protein levels and was quantified by measuring the grey value of the bands. Data are shown as means ± SD. All P values are from two‐sided t tests. B, Comparison between microarray data and qPCR results of lncRNAs associated with hypoxia. The qPCR result validated the 13 out of 210 selected lnRNAs were regulated by hypoxia, whose expression exhibited same expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP cells cultured in hypoxia/KP cells cultured in normoxia in qPCR results) in expression across the three lung adenocarcinoma tissues and KP cells cultured in hypoxia. Mean ± SD are shown, n = 3 or 2

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout

    doi: 10.1111/jcmm.14584

    Figure Lengend Snippet: Identification of hypoxia‐related lncRNAs in KP cells. A, KP cells were incubated under hypoxia with 1% O 2 and normoxia (20% O 2 ). Immunoblot (left) was used to detect HIF1α protein levels and was quantified by measuring the grey value of the bands. Data are shown as means ± SD. All P values are from two‐sided t tests. B, Comparison between microarray data and qPCR results of lncRNAs associated with hypoxia. The qPCR result validated the 13 out of 210 selected lnRNAs were regulated by hypoxia, whose expression exhibited same expression alteration direction to that in the microarray of the mouse lung adenocarcinoma tissues. The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues in microarray data of the mouse lung adenocarcinoma tissues or KP cells cultured in hypoxia/KP cells cultured in normoxia in qPCR results) in expression across the three lung adenocarcinoma tissues and KP cells cultured in hypoxia. Mean ± SD are shown, n = 3 or 2

    Article Snippet: For microarray analysis, lncRNA + mRNA mouse Gene Expression Microarray v1.0, 4x180K chip (CapitalBio Corp), was employed and conducted by CapitalBio Corp according to the manufacturer's protocols.

    Techniques: Incubation, Western Blot, Comparison, Microarray, Expressing, Transformation Assay, Cell Culture

    Confirmation of the p53‐ , Kras‐ or hypoxia‐ associated lncRNAs. A, Overlapped differentially expressed lncRNAs. B, Among the total 52 lncRNAs identified to be associated with p53 , Kras or hypoxia, 19 lncRNAs, with expression change fold greater than 3.5 in the microarray results, were selected to further confirmed by using quantitative RT‐PCR in another group of mouse lung adenocarcinoma tissues (6 lung adenocarcinoma samples and 6 normal lung tissues). The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues). The validation results of the 19 lncRNAs indicated that the microarray data correlated well with the qPCR results. Mean ± SD are shown, n = 3 or 6. C, Long noncoding RNA (lncRNA)‐lncRNA co‐expression network (LLCN) related to the 19 selected lncRNAs was constructed. Among the 19 lncRNAs, 6 lncRNAs had no significant co‐expression with other lncRNAs. The circles represent nodes of differential expressed lncRNAs (DE lncRNAs). The nodes in green colour represent the 13 remained lncRNAs. The LLCN contained a total of 4086 lncRNAs with 19 595 edges between them

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout

    doi: 10.1111/jcmm.14584

    Figure Lengend Snippet: Confirmation of the p53‐ , Kras‐ or hypoxia‐ associated lncRNAs. A, Overlapped differentially expressed lncRNAs. B, Among the total 52 lncRNAs identified to be associated with p53 , Kras or hypoxia, 19 lncRNAs, with expression change fold greater than 3.5 in the microarray results, were selected to further confirmed by using quantitative RT‐PCR in another group of mouse lung adenocarcinoma tissues (6 lung adenocarcinoma samples and 6 normal lung tissues). The heights of the columns in the chart represent the log‐transformed median fold changes (tumour sample/normal tissues). The validation results of the 19 lncRNAs indicated that the microarray data correlated well with the qPCR results. Mean ± SD are shown, n = 3 or 6. C, Long noncoding RNA (lncRNA)‐lncRNA co‐expression network (LLCN) related to the 19 selected lncRNAs was constructed. Among the 19 lncRNAs, 6 lncRNAs had no significant co‐expression with other lncRNAs. The circles represent nodes of differential expressed lncRNAs (DE lncRNAs). The nodes in green colour represent the 13 remained lncRNAs. The LLCN contained a total of 4086 lncRNAs with 19 595 edges between them

    Article Snippet: For microarray analysis, lncRNA + mRNA mouse Gene Expression Microarray v1.0, 4x180K chip (CapitalBio Corp), was employed and conducted by CapitalBio Corp according to the manufacturer's protocols.

    Techniques: Expressing, Microarray, Quantitative RT-PCR, Transformation Assay, Biomarker Discovery, Construct